Effect of Modified Simiao Granule on Type 2 Diabetic Mice and Chronic Fatty Liver Rats

CHENG Kun; LUO Tianjiong; JIANG Baoping

doi:10.16295/j.cnki.0257-358x.2018.03.021

Abstract：Objective:To explore the effect of modified Simiao Granule on blood glucose,blood lipid,and liv-er tissue in mouse with type 2 diabetic,and on the blood lipid,liver index,Lee's index and fat index in rat with chronic fatty liver. Method:(1) Type 2 diabetic mouse model was established by intraperitoneal injec-tion of streptozotocin(100 mg/kg). After the models were successfully established,mice were randomly di-vided into 6 groups:control group,model group,Simiao Granule low dose group,Simiao Granule middle dose group,Simiao Granule high dose group,and metformin group. In each group,mice were given intragastric ad-ministration of the corresponding drugs or distilled water daily. After 3 weeks ,the levels of total cholesterol (TC) and triglyceride(TG) in serum and blood glucose were measured. The liver weight index of mice was calculated and the histopathological changes were observed under optical microscope. (2) High fat emulsion was given by gavage to build chronic fatty liver rat model,and then the model rats were randomly divided into 5 groups:model group,atorvastatin group,Simiao Granule low dose group,Simiao Granule middle dose group,and Simiao Granule high dose group. A blank control group was built up beside the above groups.Each group was orally given the corresponding drugs or the same volume of distilled water. Eight weeks later,levels of TC and TG in the serum were measured. Body fat weight and liver weight were measured. The Lee's index and the fat index were calculated. Results:(1) Compared with the model group,the fasting blood glucose level signifi-cantly decreased in the metformin group and modi-fied Simiao Granule of low,middle and high dose groups(P<0.05 or P<0.01). The level of TC in the Simiao Granule of middle and high dose group was significantly lower than that in the model group (P<0.05 or P<0.01). There was no significant difference in the blood glucose,blood lipid and liver index between the metformin group and modified Simiao Granule of low,middle and high dose groups(P>0.05). Compared with the model group,no significant difference was found on steatosis index in middle and high dose groups,while there was significant difference in hydropic degeneration(P<0.01). Compared with metformin, modified Simiao Granule could obviously relieve liver tissue injury. (2) Compared with the control group, levels of TC and TG in the serum of modified Simiao Granule of the middle and high dose groups and a-torvastatin group significantly decreased(P<0.05 or P<0.01). Compared with the model group,the fat index of the low dose group was significantly lower than that of the model group(P<0.05),and moreover,the liver index and the Lee's index of the middle and high dose group and the atorvastatin group were significantly lower than those of the model group(P<0.05 or P<0.01). There was no significant difference between the Simiao groups and the atorvastatin group(P>0.05). Conclusions:Modified Simiao Granule could not only re-duce blood glucose level,but also reduce blood lipid. Compared with metformin,modified Simiao Granule can inhibit the liver enlargement of diabetic mice ,reduce the pathological changes of liver tissue. Besides ,it has some protective effect on the liver damage in type 2 diabetes mellitus.