DOI: 10.1002/ame2.12023
Regeneration of islet β-cells in tree shrews and rats
Yu-Qiong Zhao
Ya-Qian Liu
Ji-Fang Yuan
Xin Dai
Miao-Miao Niu
Xiao-Mei Sun
De-Xuan Kuang
Wen-Guang Wang
Pin-Fen Tong
Na Li
Lei Xiang
Yun-Xiao Jia
Jie-Jie Dai
Hua Chen
摘要：Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β-cells in tree shrews.Methods: In this study, we examined the changes in islet cell composition and regeneration of β-cells after streptozotocin (STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet β-cells were observed using hematoxylin and eosin (HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and PDX-1.Results: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet β-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was observed.Conclusion: The results suggest that the repair mechanism of islet β-cells in tree shrews is similar to that of humans.
机标关键词：
资助基金：the Specialized Research Fund forLaboratory Animal Science of PLA(SYDW[2014]-0009)TheNational Science and Technology Support Program(2011BAI15B01and 2014BAI01B01)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 152-161 )
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