DOI: 10.1002/ame2.12030
Comparative medical characteristics of ZDF-T2DM rats during the course of development to late stage disease
Yun-Li Pang
Jing-Wen Hu
Guang-Long Liu
Shuai-Yao Lu
摘要：Background: There are few reports on the comparative medical characteristics of type 2 diabetes models in late stage. An analysis of comparative medical characteris-tics of Zucker diabetic fatty type 2 diabetes mellitus (ZDF-T2DM) rats during the course of development to late stage disease was performed. Methods: In this study, ZDF rats were fed with high-sugar and high-fat diets to raise the fasting blood glucose, and develop of type 2 diabetes. At the late stage of T2DM, the preliminary comparative medical characteristics of the T2DM model were analyzed through the detection of clinical indicators, histopathology, related cytokine levels, and insulin-related signaling molecule expression levels. Results: In the T2DM group, the fasting blood glucose was higher than 6.8 mmol/L, the serum insulin, leptin, and adiponectin levels were significantly decreased, and glu-cose intolerance and insulin resistance were measured as clinical indicators. Regarding pathological indicators, a large number of pancreatic islet cells showed the reduction of insulin secretion, resulting in damaged glycogen synthesis and liver steatosis. At the molecular level, the insulin signal transduction pathway was inhibited by decreasing the insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), phosphatidyli-nositol 3 kinase (PI3K), and glycogen synthesis kinase 3β(GSK-3β) expression levels. Conclusion: The results show that the ZDF/T2DM rats have typical clinical, histopathological, and molecular characteristics of human T2DM and thus can be used as an effective model for T2DM drug development and treatment of advanced T2DM.
机标关键词：
资助基金：National Natural Science Foundation of China(81570749)National Natural Science Foundation of China( 81301073)National Natural Science Foundation of China( 81571254)National Science and Technology Major Project of the Ministry of Science and Technology of China(2017ZX09101003-003-008)Yunnan Natural Science Foundation(2013FB089)the Fundamental Research Funds for the Central Universities(33320140083)the CAMS Initiative for Innovative Medicine (2016-I2M-2-006)The Special Funds for High-level Health and Family Planning Technical Personnel training of Yunnan Province(D-201653)The Special Funds for High-level Health and Family Planning Technical Personnel training of Yunnan Province( 2017HB068)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 203-211 )
英文信息