DOI: 10.1002/ame2.12038
The pattern of plasma BCAA concentration and liver Bckdha gene expression in GK rats during T2D progression
Wenlu Zhang
Yu'e Wu
Wei Fan
Hongmei Chen
Hongli Du
Junhua Rao
摘要：Background:This study was conducted to measure the concentration of branched chain amino acid (BCAA) in different species and detect the expression pattern of the liver Bckdha gene in Goto-Kakizaki (GK) rats during type 2 diabetes (T2D) progression.Methods:We measured the concentration of BCAA in GK rats,induced T2D cynomolgus monkeys and T2D humans by liquid chromatography tandem mass spectrometry,and used real-time quantitative PCR to analyze the gene expression of Bckdha and Bckdk,which encode the rate-limiting enzymes in catabolism of,respectively,branched chain amino acids and branched chain α-keto acid dehydrogenase kinase.Results:In this study,we showed that GK rat BCAA concentrations were significantly reduced at 4 and 8 weeks (P ＜ 0.05 and P ＜ 0.01,respectively),while the expression of Bckdha in GK rat liver was increased at 4 and 8 weeks (1.62-fold and 1.93-fold,respectively).The BCAA concentrations were significantly reduced in dietinduced T2D cynomolgus monkeys (P ＜ 0.01),but significantly increased in T2D humans (P ＜ 0.001).Conclusions:Our results showed that BCAA concentrations changed at different times and by different amounts in different species and during different periods of T2D progress,and the significant changes of BCAA concentration in the three species indicated that BCAA might participate in the progress of T2D.The results suggested that the increased expression of Bckdha in GK rat liver might partially explain the reduced plasma BCAA concentration at 4 and 8 weeks.Further studies are required to investigate the exact mechanism of BCAA changes in non-obese T2D.
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资助基金：grants from the Science and Technology Program of Guangdong province(2014B070706020)the National Science and Technology Support Program(2014BAI03B00)the National Science and Technology Support Program(2015BAI07B0)the Science and Technology Program of Guangzhou,China(201508020040)the Science and Technology Program of Guangzhou,China(201707010330)the S&T Development Project of Guangdong Academy of Science(2017A070702014)
论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 305-313 )
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