DOI: 10.1002/ame2.12056
Low-level laser therapy enhances the number of osteocytes in calvaria bone defects of ovariectomized rats
Priscilla Hakime Scalize
Luiz Gustavo de Sousa
Lígia Maria Napolitano Gon(c)alves
Dimitrius Leonardo Pitol
Marcelo Palinkas
Ant(o)nio Augusto Coppi
Mariah Acioli Righeti
Vitória Ricardo
Karina Fittipaldi Bombonato-Prado
Simone Cecílio Hallak Regalo
Selma Siessere
摘要：Background:Osteoporosis can make bone repair difficult.Low-level laser therapy (LLLT) has been shown to be a promising tool for bone neoformation.This study aimed to analyze the effect of LLLT on calvaria bone defects of ovariectomized rats using stereology.Methods:Fifty-four Wistar rats were subjected to bilateral ovariectomy,and bone defects were created in calvaria after 150 days.The animals were divided into nine groups (n =6 per group),and 24 hours after the bone defects were created they received three,six or 12 sessions of LLLT at 0,20 or 30 J/cm2,using a 780-nm low-intensity GaAIAs laser.One-way ANOVA followed by Tukey's post hoc test was used for data processing.A difference of P ＜ 0.05 was considered statistically significant.The parameters evaluated were osteocyte density (Nvost),total osteocyte number (Ntoost),trabecular surface density (Svt),and trabecular surface area (Sat).Results:Data obtained showed that Ntoost,Svt,and Sat in group G2 rats were significantly different from G1 (0 J/cm2) (P ＜ 0.05).Compared to group G4,G5 presented higher values for the parameters Svt and Sat,and G6 presented significantly higher values for almost all the analyzed parameters (NVost,Ntoost,Svt,and Sat) (P ＜ 0.05).Compared to group G7,G8 showed a higher value only for the parameter Sat,and G9 showed significantly higher values for parameters NVost,Ntpost,Svt,and Sat.Conclusion:We conclude that LLLT stimulated bone neoformation and contributed to an increase in the total number of osteocytes,especially with a laser energy density of 30 J/cm2 given for six and 12 sessions.
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论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 51-57 )
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