DOI: 10.1002/ame2.12050
Orthotopic induction of CH157MN convexity and skull base meningiomas into nude mice using stereotactic surgery and MRI characterization
Francesca La Cava
Alberto Fringuello Mingo
Pietro Irrera
Aldo Di Vito
Alessia Cordaro
Chiara Brioschi
Sonia Colombo Serra
Claudia Cabella
Enzo Terreno
Luigi Miragoli
摘要：Meningioma in vivo research is hampered by the difficulty of establishing an easy and reproducible orthotopic model able to mimic the characteristics of a human meningioma.Moreover,leptomeningeal dissemination and high mortality are often associated with such orthotopical models,making them useless for clinical translation studies.An optimized method for inducing meningiomas in nude mice at two different sites is described in this paper and the high reproducibility and low mortality of the models are demonstrated.Skull base meningiomas were induced in the auditory meatus and convexity meningiomas were induced on the brain surface of 23 and 24 nude mice,respectively.Both models led to the development of a mass easily observable by imaging methods.Dynamic contrast enhanced MRI was used as a tool to monitor and characterize the pathology onset and progression.At the end of the study,histology was performed to confirm the neoplastic origin of the diseased mass.
机标关键词：
资助基金：Human meningioma cells(CH157MN)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 58-63 )
英文信息