DOI: 10.1002/ame2.12053
A novel mouse model of adult T-cell leukemia cell invasion into the spinal cord
Takeo Ohsugi
Shuhei Tanaka
Keigo Iwasaki
Yusuke Nagano
Tomohiro Kozako
Kazuya Matsuda
Takuya Hirose
Kazushige Takehana
摘要：Adult T-cell leukemia (ATL) is a mature T-cell malignancy caused by human T-cell leukemia virus type Ⅰ infection,and 10％-25％ of patients show central nervous system (CNS) involvement.CNS involvement significantly reduces survival and there are no effective treatments for CNS involvement.Therefore,an appropriate animal model is required to evaluate the inhibitory effects of novel drugs on the progression of ATL with CNS involvement.Here,we established a mouse model of ATL with CNS involvement using NOD.Cg-Prkdcscidll2rgtm1wjl/SzJ mice inoculated with ATL cells intramuscularly in the postauricular region,and these mice showed paraparesis.Of the 10 mice inoculated with ATL cells intramuscularly (I.M.) at 5 weeks of age,8 (80％)showed paraparesis,whereas none of the 10 mice inoculated with ATL cells subcutaneously (S.C.) showed paraparesis.In the I.M.group,PCR detected HTLV-1-specific genes in the thoracic and lumbar vertebrae;however,in the S.C.group,the vertebrae were negative for HTLV-1 genes.Histological analysis revealed a particularly high incidence of tumors,characterized by accumulation of the injected cells,in the thoracic vertebrae of mice in the I.M.group.Tumor cell infiltration was relatively high in the bone marrow.Spinal cord compression caused by invasion of the tumor mass outside the pia mater was observed in the thoracic vertebrae of the spinal cord.In conclusion,we have reported a mouse model of tumor growth with paraparesis that may be used to assess novel therapeutic agents for ATL with CNS involvement.
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资助基金：Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science,Grant/Award Number(No.24500493)
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 64-67 )
英文信息