Allantoin attenuates deficits of behavioural and motor nerve conduction in an animal model of cisplatin-induced neurotoxicity in rats

Vindya N S; Aqib Mohamad; Rema Razdan

doi:10.1002/ame2.12070

摘要：Background:The aim of the present study was to evaluate the neuroprotective effect of allantoin in cisplatin-induced toxicity in rats.Methods:Adult male Wistar rats weighing 160-200 g were used.Neuropathy was induced by injecting cisplatin (2 mg/kg,ip,twice a week for 6 weeks) and the rats were concurrently treated with allantoin (200 and 400 mg/kg,po) for 8 weeks.At the end of the study,body weight and hemogram were measured.Behavioural tests were performed,including tests for cold and hot hyperalgesia,motor co-ordination,locomotor activity,mechano-tactile allodynia and mechanical hyperalgesia.The rats were then sacrificed and sciatic nerve conduction velocity was determined.The antioxidant enzyme and nitric oxide levels in sciatic nerve homogenates were measured.Results:In this study,allantoin restored the motor nerve conduction velocity deficits induced by cisplatin,and the allantoin-treated rats showed improvement in cold and thermal hyperalgesia,mechano-tactile allodynia,and mechanical hyperalgesia.Allantoin treatment also improved the rats' hematological status,increasing haemoglobin,platelet and RBC counts compared to the cisplatin-treated group.Allantoin treatment also mitigated the functional abnormalities seen in the cisplatin neuropathy group,protecting neurons from the neurotoxic effects of cisplatin.Conclusion:Allantoin shows promise for use as an adjuvant drug in cancer treatment to protect against cisplatin-induced neuropathy.