DOI: 10.1002/ame2.12057
Lgr5+ intestinal stem cell sorting and organoid culture
Ruixue Liu
Haifeng Li
Juan Cai
Qiang Wei
Xiaonan Han
摘要：Intestinal epithelial stem cells (IESCs) are one of the most rapidly self-renewing and proliferating adult stem cells.The IESCs reside at the bottom of intestinal and colonic crypts,giving rise to all intestinal epithelial lineages and maintaining intestinal epithelial replenishment.The technique of three-dimensional culture based upon intestinal stem cell biology has been recently developed to study gastrointestinal development and disease pathogenesis.Here,we summarize the techniques used to isolate Lgr5-positive IESCs to form the enteroids from intestine or colonoids from colon,and present the means to examine these organoid functions.This study will provide a simple and practical way for producing intestinal tissues in the laboratory.
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论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 132-135 )
英文信息