DOI: 10.1002/ame2.12084
Characterization of inflammation and insulin resistance in high-fat diet-induced male C57BL/6J mouse model of obesity
Dimiter Avtanski
Valentin A. Pavlov
Kevin J. Tracey
Leonid Poretsky
1.Lenox Hill Hospital, Friedman Diabetes Institute, Northwell Health, New York, NY,USA;The Feinstein Institutes for Medical Research, Northwell Health, Manhasset,NY, USA;Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead,NY, USA2.The Feinstein Institutes for Medical Research, Northwell Health, Manhasset,NY, USA;Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead,NY, USA
摘要：Background: Animal models of diet-induced obesity (DIO) are commonly used in medical research for mimicking human diseases. There is no universal animal model, and careful evaluation of variety of factors needs to be considered when designing new experiments. Here, we investigated the effect of 9 weeks high-fat diet (HFD) intervention, providing 60％ energy from fat, on parameters of inflammation and in-sulin resistance in male C57BL/6J mice. Methods: Six weeks old mice were initiated on regular diet (RD) or HFD providing 60 kcal energy from fat for 9 weeks. Fasting blood glucose levels were measured by glucometer, and fasting plasma levels of insulin and proinflammatory cytokines by Luminex assay. Insulin sensitivity was evaluated by using QUICKI and HOMA2 indexes. Results: HFD mice showed ～ 40％ higher body weight and ～ 20％ larger abdominal circumference, due to an increase in the white adipose tissue mass. Liver examination revealed increased size and higher hepatic lipid accumulation in livers from HFD mice compared to their RD counterparts. Animals from the HFD group were characterized with significantly higher presence of crown-like structures (CLS) in WAT and higher plasma levels of proinflammatory cytokines (TNF-α, IL-6, leptin, MCP-1, PAI-1, and resistin). HFD-fed mice also demonstrated impaired insulin sensitivity (lower QUICKI, higher HOMA-insulin resistance (HOMA-IR), and lower HOMA-percent sensitivity (HOMA-％S)) index values. Conclusion: Male C57BL/6J mice on 9 weeks HFD providing 60 kcal energy from fat display impaired insulin sensitivity and chronic inflammation, thus making this DIO mouse model appropriate for studies of early stages of obesity-related pathology.
机标关键词：insulin resistanceinsulin sensitivityproinflammatory cytokineschronic inflammationblood glucose levelslipid accumulationadipose tissueanimal model
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 252-258 )
英文信息