DOI: 10.1002/ame2.12089
A simple method for inducing nonalcoholic steatohepatitis with fibrosis
Leyla Yahaghi
Azadeh Ebrahim-Habibi
Nasim Hayati-Roodbari
Shiva Irani
Parichehreh Yaghmaei
1.Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran2.Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran;Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran
摘要：Background: Nonalcoholic fatty liver disease (NAFLD) is increasingly occurring in sedentary people, and may progress to NASH and hepatocellular carcinoma. It is es-sential to design affordable animal models for the study of various diseases, including fatty liver, which was the aim of the study. In this study, a high-fat diet was devised that triggers NASH's animal model quickly and easily. High-fat diet (HFD) was used both with intra-mouth oral gavage and in combination with animal pellets. Methods: Twenty-four male C57BL/6J mice were divided into HFD and ND groups, which received a high-fat diet and a normal diet, respectively. At the end of the ex-periment (fourth week of treatment), body and liver weights, biochemical param-eters, PPAR-α gene expression and histopathologic characteristics of the liver were evaluated. Results: During 4 weeks, body weight of mice did not show a significant increase in the HFD group compared to the ND group, while weight gain of the liver was signifi-cant. Histological assessment of the HFD group's liver confirmed NASH symptoms. In the HFD group, HDL-c, SOD, catalase, FRAP, adiponectin, and PPAR-α decreased significantly, and lipid profiles, hepatic enzymes, MDA, leptin, and TNF-α showed a significant increase compared to the ND group. Conclusion: Our high-fat diet has successfully induced all aspects of NASH with fi-brosis in 4 weeks, and with low cost.
机标关键词：hepatocellular carcinomafatty liverliver diseaseanimal modelsweight gainlow cost
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 282-290 )
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