DOI: 10.1002/ame2.12087
Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta)
Jose J. Rosado-Franco
Marcos J. Ramos-Benitez
Laura M. Parodi
Derick Rosario
Nicole Compo
Luis D. Giavedoni
Ana M. Espino
1.Department of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico2.Southwest National Primate Research Center, Texas Biomedical Research Institute,San Antonio, TX, USA3.Chemistry Department, Rio Piedras Campus, University of Puerto Rico, San Juan, Puerto Rico4.Medical Sciences Campus, Caribbean Primate Research Center University of Puerto Rico, Toa Baja, Puerto Rico
摘要：The aim of this study was to identify inflammation-associated markers during the early phase of sepsis in rhesus macaque. Four rhesus macaques were given an in-travenous dose of 1010 CFU/kg of E. coli. Blood samples were collected before, or 30 minutes, 2, 4, 6 and 8 hours after E. coli infusion. Physiological parameters, bac-teremia, endotoxemia, C-reactive protein (CRP), procalcitonin (PCT), and plasma cy-tokines/chemokines were determined for each animal. Bacteremia was present in all animals from 30 minutes to 3 hours after E. coli infusion whereas endotoxin was de-tected during the full-time course. CRP and PCT levels remained at detectable levels during the whole experimental window suggesting an ongoing inflammatory process. Signature cytokines and chemokines such as TNF-α, MIP-1α, and MIP-1β peaked about 2 hours after E. coli infusion and decreased thereafter. Plasma IL-6, IL-12p40, IFN-γ, and IL-1Ra, as well as I-TAC, MIG, IP-10 and MCP-1, remained at detectable levels after 4 hours of E. coli infusion. This nonhuman primate model could be useful for the assessment of new therapeutics aiming to suppress key inflammatory markers throughout sepsis early phases.
机标关键词：rhesus macaqueearly phases
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 326-333 )
英文信息