DOI: 10.1002/ame2.12095
Screening high-quality fetal bovine serum for porcine oocyte maturation in vitro
Xueqing Liu
Qiaoli Lang
Meng Wu
Xiaoyan You
Qiling He
Ling Luo
Zijia Liu
Puying Xiao
Nan Huang
Xi Yang
Liangpeng Ge
1.Chongqing Academy of Animal Sciences,Chongqing, China;Key Laboratory of Pig Industry Sciences,Ministry of Agriculture, Chongqing, China;Key Laboratory of Pig Industry Sciences,Chongqing, China;Technical Engineering Center for the Development and Utilization of Medical Animal Resources, Chongqing, China2.Chongqing Academy of Animal Sciences,Chongqing, China
摘要：Fetal bovine serum (FBS) is widely used in cell cultures due to its high stability and easy access. It was also used as a substitute for porcine follicular fluid (PFF) in pre-vious studies. However, FBS components are unclear, and the presence of FBS in culture media may introduce a variation from batch to batch. This study aimed to establish an effective method to screen FBS in place of PFF in the culture media for porcine oocytes in vitro. We screened FBS from different sources by using por-cine fetal fibroblast cells. The effects of six FBS samples on porcine fetal fibroblast cell growth were tested via frozen cell survival assay, cell clone formation assay, cell growth curve, and cell passage activity assay. The best serum that we called GFBS (heat-inactivated FBS, cat. no. 10500-64; Gibco) showed a similar effect on the matu-ration and development of porcine oocytes to that of PFF and can be used as a good substitute for PFF. These results suggested that the porcine fetal fibroblast cell cul-ture test can be used as a valuable method to screen FBS for porcine oocyte matura-tion and embryonic development in vitro.
机标关键词：maturation in vitroculture mediacell growthembryonic developmentfollicular fluidfibroblast cellsporcine oocyteactivity assay
论文发表日期：2019-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 334-339 )
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