DOI: 10.1002/ame2.12099
Hematological reference values and animal welfare parameters of BALB/C-FMABC (Mus musculus) inoculated with Ehrlichtumor kept in the vivarium at ABC Medical School
Patrick Gabriel dos Santos Pessini1
Paula Regina Knox de Souza2
Camila dos Santos Chagas3
Emily Garcia Sampaio3
Daniel Santos Neves3
Giuliana Petri3
Fernando Luiz Affonso Fonseca3
Emerson Barbosa da Silva3
1.Clinical Analysis Laboratory of the ABCUniversity Health Center, Santo André, Brazil;Health Sciences Institute/Paulista University - UNIP, S?o Paulo, Brazil2.Health Sciences Institute/Paulista University - UNIP, S?o Paulo, Brazil3.Clinical Analysis Laboratory of the ABCUniversity Health Center, Santo André, Brazil
摘要：Background: Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs, and can be recorded to create reference values used for clinical diagnosis of diseases. Many research laboratories lack the means to establish their own set of reference parameters for use in their research, and while there are articles in the literature that discuss laboratory parame-ters for healthy BALB/c mice, few studies address the evaluation of these parameters in pathological situations, such as in mice inoculated with Ehrlich tumor.Method: BALB/c-FMABC mice previously inoculated with Ehrlich tumor were main-tained under appropriate conditions. Blood samples were taken for analysis of hema-tological parameters using automated and semi-automated equipment to create a set of the animal welfare parameters for evaluation. Result: Results were obtained for all the hematological parameters for all groups analyzed. These showed: statistically significant differences between the initial and final tumor weight; comparable initial tumour volume and weight; an increase in leu-kocytes in the 7-day group with a characteristic predominance of lymphocytes and neutrophils; statistically significant changes in RDW in the 21-day group and in the welfare parameters in the 28-day group. Conclusion: The study successfully defined and established reference values for he-matological and welfare parameters for all groups analyzed.
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论文发表日期：2020-03-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 32-39 )
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