DOI: 10.1002/ame2.12109
Induction of diabetes in cynomolgus monkey with one shot of analytical grade streptozotocin
Zhengzhao Liu1
Ying Lu2
Wenbao Hu2
Hidetaka Hara3
Yifan Dai4
Zhiming Cai2
Lisha Mou2
1.Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, China;Movement System Injury and Repair Research Center, Xiangya Hospital of Central South University, Changsha, China2.Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, China3.Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, China4.Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, Birmingham, AL, USA
摘要：Backgrounds: Streptozotocin (STZ)- induced diabetic monkey is a wide used preclini-cal animal model for the investigation of diabetes such as islet transplantation and development of diabetic drugs. There are serious side effects of this method, includ-ing nausea, emesis, weight loss, liver damage, renal failure, and metabolic acidosis. In order to reduce the side effects, diabetic monkeys were induced using clinical-grade STZ. However, clinical-grade STZ is not available in China. Here, we establised a method by using 100 mg/kg analytical-grade STZ to induce complete diabetes in cynomolgus monkey without generating adverse effects to liver and renal.Methods: Three cynomolgus monkeys were used in this study. 100 mg/kg STZ dis-solved in normal saline and infused through vein line in 5 minutes after indwelling catheter in the carotid artery and jugular vein. After the STZ administration, blood glucose levels were examined every 1 or 2 hours in the first 48 hours. Then, blood glucose levels were examined twice per day during the first week after the STZ in-jection. Insulin and C-peptide levels were measured by ELISA. Blood chemistry of hepatic and renal function tests were performed. Insulin and glucagon expression in the islet of diabetic monkey and normal monkey were examined by immunohisto-chemistry assays. Results: The stimulated C-peptide level (Intravenous glucose tolerance test) which is less than 0.5 ng/mL, the triphasic blood glucose response and the destroyed β cell suggested the complete induction of diabetes model. No apparent adverse effects were observed including no signs of vomiting and toxicity after STZ injection. Conclusion: In summary, we established a safe and reproducible STZ-induced dia-betic cynomolgus monkey model for islet transplantation which will be used to de-velop novel approaches for the treatment of diabetes.
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论文发表日期：2020-03-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 79-86 )
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