DOI: 10.1002/ame2.12108
Age-related rhesus macaque models of COVID-19
Pin Yu
Feifei Qi
Yanfeng Xu
Fengdi Li
Peipei Liu
Jiayi Liu
Linlin Bao
Wei Deng
Hong Gao
Zhiguang Xiang
Chong Xiao
Qi Lv
Shuran Gong
Jiangning Liu
Zhiqi Song
Yajin Qu
Jing Xue
Qiang Wei
Mingya Liu
Guanpeng Wang
Shunyi Wang
Haisheng Yu
Xing Liu
Baoying Huang
Wenling Wang
Li Zhao
Huijuan Wang
Fei Ye
Weimin Zhou
Wei Zhen
Jun Han
Guizhen Wu
Qi Jin
Jianwei Wang
Wenjie Tan
Chuan Qin
摘要：Background: Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, has become a public health emergency of international concern. The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models. Methods: Three 3-5 years old and two 15 years old rhesus macaques were intratra-cheally infected with SARS-CoV-2, and then analyzed by clinical signs, viral replica-tion, chest X-ray, histopathological changes and immune response. Results: Viral replication of nasopharyngeal swabs, anal swabs and lung in old mon-keys was more active than that in young monkeys for 14 days after SARS-CoV-2 chal-lenge. Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema, notably, old monkeys exhibited diffuse severe interstitial pneumonia. Viral antigens were detected mainly in alveolar epithelial cells and macrophages. Conclusion: SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys. Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection.
机标关键词：
论文发表日期：2020-03-25
页数：5( 93-97 )
英文信息