DOI: 10.1002/ame2.12112
Assessing neuraxial microstructural changes in a transgenic mouse model of early stage Amyotrophic Lateral Sclerosis by ultra-high field MRI and diffusion tensor metrics
Rodolfo G.Gatto1
Carina Weissmann2
Manish Amin3
Ariel Finkielsztein4
Ronen Sumagin4
Thomas H.Mareci3
Osvaldo D.Uchitel2
Richard L.Magin1
1.Department of Bioengineering,University of Illinois at Chicago,Chicago,IL,USA2.Instituto de Fisiología Biologia Molecular y Neurociencias-IFIBYNE-CONICET,University of Buenos Aires,Buenos Aires,Argentina3.Department of Biochemistry,National High Magnetic Field Laboratory,University of Florida,Gainesville,FL,USA4.Department of Pathology,School of Medicine,Northwestern University,Chicago,IL,USA
摘要：Objective:Cell structural changes are one of the main features observed during the development of amyotrophic lateral sclerosis (ALS).In this work,we propose the use of diffusion tensor imaging (DTI) metrics to assess specific ultrastructural changes in the central nervous system during the early neurodegenerative stages of ALS.Methods:Ultra-high field MRI and DTI data at 17.6T were obtained from fixed,ex-cised mouse brains,and spinal cords from ALS (G93A-SOD1) mice.Results:Changes in fractional anisotropy (FA) and linear,planar,and spherical ani-sotropy ratios (CL,CP,and CS,respectively) of the diffusion eigenvalues were meas-ured in white matter (WM) and gray matter (GM) areas associated with early axonal degenerative processes (in both the brain and the spinal cord).Specifically,in WM structures (corpus callosum,corticospinal tract,and spinal cord funiculi) as the dis-ease progressed,FA,CL,and CP values decreased,whereas CS values increased.In GM structures (prefrontal cortex,hippocampus,and central spinal cord) FA and CP decreased,whereas the CL and CS values were unchanged or slightly smaller.Histological studies of a fluorescent mice model (YFP,G93A-SOD1 mouse) corrobo-rated the early alterations in neuronal morphology and axonal connectivity measured by DTI.Conclusions:Changes in diffusion tensor shape were observed in this animal model at the early,nonsymptomatic stages of ALS.Further studies of CL,CP,and CS as im-aging biomarkers should be undertaken to refine this neuroimaging tool for future clinical use in the detection of the early stages of ALS.
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论文发表日期：2020-06-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 117-129 )
英文信息