DOI: 10.1002/ame2.12114
A segmental defect adaptation of the mouse closed femur fracture model for the analysis of severely impaired bone healing
Amandeep Kaur1
Subburaman Mohan2
Charles H.Rundle3
1.Musculoskeletal Disease Center,Research Service 151,Jerry L.Pettis Memorial Veterans Administration Medical Center,Loma Linda,CA,USA2.Musculoskeletal Disease Center,Research Service 151,Jerry L.Pettis Memorial Veterans Administration Medical Center,Loma Linda,CA,USA;Department of Medicine,Loma Linda University,Loma Linda,CA,USA;Department of Orthopedic Surgery,Loma Linda University,Loma Linda,CA,USA3.Musculoskeletal Disease Center,Research Service 151,Jerry L.Pettis Memorial Veterans Administration Medical Center,Loma Linda,CA,USA;Department of Medicine,Loma Linda University,Loma Linda,CA,USA
摘要：Objective:To better characterize nonunion endochondral bone healing and evaluate novel therapeutic approaches for critical size defect healing in clinically challenging bone repair,a segmental defect model of bone injury was adapted from the three-point bending closed fracture technique in the murine femur.Methods:The mouse femur was surgically stabilized with an intramedullary threaded rod with plastic spacers and the defect adjusted to different sizes.Healing of the dif-ferent defects was analyzed by radiology and histology to 8 weeks postsurgery.To determine whether this model was effective for evaluating the benefits of molecular therapy,BMP-2 was applied to the defect and healing then examined.Results:Intramedullary spacers were effective in maintaining the defect.Callus bone formation was initiated but was arrested at defect sizes of 2.5 mm and above,with no more progress in callus bone development evident to 8 weeks healing.Cartilage development in a critical size defect attenuated very early in healing without bone development,in contrast to the closed femur fracture healing,where callus cartilage was replaced by bone.BMP-2 therapy promoted osteogenesis of the resident cells of the defect,but there was no further callus development to indicate that healing to pre-surgery bone structure was successful.Conclusions:This segmental defect adaptation of the closed femur fracture model of murine bone repair severely impairs callus development and bone healing,reflecting a challenging bone injury.It is adjustable and can be compared to the closed fracture model to ascertain healing deficiencies and the efficacy of therapeutic approaches.
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论文发表日期：2020-06-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 130-139 )
英文信息