DOI: 10.1002/ame2.12119
Curculigo recurvata W.T.Aiton exhibits anti-nociceptive and anti-diarrheal effects in Albino mice and an in silico model
Shabbir Ahmad1
Mst.Samima Nasrin2
A.S.M.Ali Reza2
Nishan Chakrabarty1
Md.Akramul Hoque1
Sanjida Islam1
Mohammad Shah Hafez Kabir3
Syed Mohammed Tareq1
A.H.M.Khurshid Alam4
Md.Areeful Haque5
Md.Saiful Islam Arman1
1.Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh2.Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh;Department of Biochemistry & Molecular Biology,University of Chittagong,Chittagong,Bangladesh3.Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh;Department of Chemistry,Wayne State University,Detroit,MI,USA4.Department of Pharmacy,University of Rajshahi,Rajshahi,Bangladesh5.Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh;Drug and Herbal Research Centre,Faculty of Pharmacy,Universiti Kebangsaan Malaysia,Kuala Lumpur,Malaysia
摘要：Background:Curculigo recurvata (C.recurvata) is an enthnomedicinally important herb reported to have significant medicinal values.The present study aimed to explore the in vivo and in silico anti-nociceptive and anti-diarrheal effects of a C.recurvate rhi-zome methanol extract (Me-RCR).Methods:The analgesic effects of Me-RCR were assessed using acetic acid-induced writhing and the formalin-induced flicking test.The drugs were administered intra-peritoneally (IP) at doses of 200 and 400 mg/kg body weight (bw).Anti-diarrheal activity was evaluated by assessing intestinal motility,hypersecretion,and fecal score in mice at oral doses of 200 and 400 mg/kg·bw.Computer facilitated analyses for anti-nociceptive and anti-diarrheal activities of three isolated compounds from C.recurvata were undertaken to identify the best-fit phytoconstituents.Results:The Me-RCR showed significant (P < .05) peripheral anti-nociception at the highest dose.The extract inhibited both early and late phases of nociception in the formalin-induced writhing test.In the castor oil-induced diarrhoea model,the extract significantly (P < .05) prolonged the onset time of diarrhoea,inhibited percentage of diarrhoea,and decreased both the volume and weight of intestinal contents.Rates of intestinal fluid accumulation inhibition were (33.61 ± 1.00)％ and (46.44 ± 0.89)％ at Me-RCR doses of 200 and 400 mg/kg·bw,respectively.Moreover,a significant (P < .05) reduction in gastrointestinal motility was observed.An absorption,distribution,metab-olism,excretion and/or toxicity (ADME/T) test showed that the selected compounds yielded promising results,satisfying Lipinski's rule of five for predicting drug-like poten-tial.Notably,of the three phytoconstituents curculigine and isocurculigine possessed the highest affinity for the COX-1 and COX-2.Isocurculigine was also identified as the most effective anti-diarrheal compound in the computer-facilitated model.Conclusion:An extract of the plant C.recurvata showed potential analgesic and anti-diarrheal activity due to the presence of one or more active secondary metabolite(s).
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论文发表日期：2020-06-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 169-181 )
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