DOI: 10.1002/ame2.12139
Clinical hematological and biochemical parameters in Swiss, BALB/c, C57BL/6 and B6D2F1 Mus musculus
1.Health Sciences Center,Institute of Microbiology Paulo de Góes,Federal University of Rio de Janeiro,Rio de Janeiro,RJ,Brazil;Laboratory of Diphtheria and Corynebacteria of Clinical Relevance,Faculty of Medical Sciences,University of the State of Rio de Janeiro,Rio de Janeiro,RJ,Brazil;The Collaborating Centre for Reference and Research on Diphtheria/National Health Foundation/Ministry of Health,Ri2.Laboratory of Clinical Research and Molecular Diagnostic Prof. Marcílio Dias do Nascimento,Department of Clinical Practice and Pathology,Fluminense Federal University,Niterói,RJ,Brazil3.Laboratory of Diphtheria and Corynebacteria of Clinical Relevance,Faculty of Medical Sciences,University of the State of Rio de Janeiro,Rio de Janeiro,RJ,Brazil;The Collaborating Centre for Reference and Research on Diphtheria/National Health Foundation/Ministry of Health,Rio de Janeiro,RJ,Brazil4.Laboratory Bacterial Vaccines,Department of Immunology,National Institute for Quality Control in Health,Oswaldo Cruz Foundation,Rio de Janeiro,RJ,Brazil5.Institute of Science and Technology in Biomodels,Oswaldo Cruz Foundation,Rio de Janeiro,RJ,Brazil6.Institute of Biology,Federal Fluminense University,Niterói,RJ,Brazil
摘要：Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these labora-tory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice. Methods:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the sys-tems pocH-100iV Diff ? for 19 hematological parameters and VITROS ? 350 for 12 biochemical parameters. Results:Results are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability. Conclusion:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.
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论文发表日期：2020-12-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 304-315 )
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