DOI: 10.1002/ame2.12151
Studying host genetic background effects on multimorbidity of intestinal cancer development, type 2 diabetes and obesity in response to oral bacterial infection and high-fat diet using the collaborative cross (CC) lines
Asal Milhem1
Hanifa J.Abu Toamih- Atamni1
Luna Karkar1
Yael Houri- Haddad2
Fuad A.Iraqi1
1.Department of Clinical Microbiology and Immunology,Sackler Faculty of Medicine,Tel- Aviv University,Tel Aviv,Israel2.Department of Prosthodontics,Dental School,The Hebrew University,Hadassah Jerusalem,Israel
摘要：Background:Multimorbidity of intestinal cancer (IC), type 2 diabetes (T2D) and obe-sity is a complex set of diseases, affected by environmental and genetic risk factors. High- fat diet (HFD) and oral bacterial infection play important roles in the etiology of these diseases through inflammation and various biological mechanisms. Methods:To study the complexity of this multimorbidity, we used the collaborative cross (CC) mouse genetics reference population. We aimed to study the multimor-bidity of IC, T2D, and obesity using CC lines, measuring their responses to HFD and oral bacterial infection. The study used 63 mice of both sexes generated from two CC lines (IL557 and IL711). For 12 weeks, experimental mice were maintained on spe-cific dietary regimes combined with co- infection with oral bacteria Porphyromonas gingivalis and Fusobacterium nucleatum , while control groups were not infected. Body weight (BW) and results of a intraperitoneal glucose tolerance test (IPGTT) were re-corded at the end of 12 weeks, after which length and size of the intestines were assessed for polyp counts. Results:Polyp counts ranged between 2 and 10 per CC line. The combination of HFD and infection significantly reduced ( P < .01) the colon polyp size of IL557 females to 2.5 cm 2 , compared to the other groups. Comparing BW gain, IL557 males on HFD gained 18 g, while the females gained 10 g under the same conditions and showed the highest area under curve (AUC) values of 40000- 45000 (min mg/dL) in the IPGTT. Conclusion:The results show that mice from different genetic backgrounds respond differently to a high fat diet and oral infection in terms of polyp development and glucose tolerance, and this effect is gender related.
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论文发表日期：2021-03-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 27-39 )
英文信息