DOI: 10.1002/ame2.12164
Group A streptococcal antigen exposed rat model to investigate neurobehavioral and cardiac complications associated with post- streptococcal autoimmune sequelae
Rukshan A.M.Rafeek1
Catherine M.Lobbe1
Ethan C.Wilkinson1
Adam S.Hamlin1
Nicholas M.Andronicos1
David J.McMillan2
Kadaba S.Sriprakash3
Natkunam Ketheesan1
1.School of Science&Technology,University of New England,Armidale,NSW,Australia2.School of Science&Technology,University of New England,Armidale,NSW,Australia;School of Science,Technology,Engineering and Genecology Research Centre,University of the Sunshine Coast,Maroochydore DC,QLD,Australia3.School of Science&Technology,University of New England,Armidale,NSW,Australia;QIMR Berghofer Medical Research Institute,Herston,QLD,Australia
摘要：Background : The neuropsychiatric disorders due to post- streptococcal autoimmune complications such as Sydenham' s chorea (SC) are associated with acute rheumatic fever and rheumatic heart disease (ARF/RHD). An animal model that exhibits char-acteristics of both cardiac and neurobehavioral defects in ARF/RHD would be an important adjunct for future studies. Since age, gender, strain differences, and geno-types impact on the development of autoimmunity, we investigated the behavior of male and female Wistar and Lewis rat strains in two age cohorts ( <6 weeks and> 12 weeks) under normal husbandry conditions and following exposure to group A streptococcus (GAS). Methods : Standard behavioral assessments were performed to determine the impair-ments in fine motor control (food manipulation test), gait and balance (beam walk-ing test), and obsessive- compulsive behavior (grooming and marble burying tests). Furthermore, electrocardiography, histology, and behavioral assessments were per-formed on male and female Lewis rats injected with GAS antigens. Results : For control Lewis rats there were no significant age and gender dependent differences in marble burying, food manipulation, beam walking and grooming be-haviors. In contrast significant age- dependent differences were observed in Wistar rats in all the behavioral tests except for food manipulation. Therefore, Lewis rats were selected for further experiments to determine the effect of GAS. After ex-posure to GAS, Lewis rats demonstrated neurobehavioral abnormalities and cardiac pathology akin to SC and ARF/RHD, respectively. Conclusion : We have characterised a new model that provides longitudinal stability of age- dependent behavior, to simultaneously investigate both neurobehavioral and cardiac abnormalities associated with post- streptococcal complications.
机标关键词：
论文发表日期：2021-06-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 151-161 )
英文信息