DOI: 10.1002/ame2.12175
Microarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer's disease
Zhiqi Song
Yajin Qu
Yanfeng Xu
Ling Zhang
Li Zhou
Yunlin Han
Wenjie Zhao
Pin Yu
Yu Zhang
Xianglei Li
Chuan Qin
Key Laboratory of Human Disease Comparative Medicine,Chinese Ministry of Health,Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Beijing,China
摘要：Background: Alzheimer ' s disease (AD) is an incurable and irreversible neurodegen-erative disease, without a clear pathogenesis. Therefore, identification of candidates before amyloid- β plaque (Aβ) deposition proceeds is of major significance for earlier intervention in AD.Methods: To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model, microRNAs (miRNAs) from urinary exosomes in 1- month- old pre- Aβ accumulation 5XFAD mice models and their littermate controls were profiled by mi-croarray analysis. The differentially expressed miRNAs were further analyzed via droplet digital PCR (ddPCR). Results: Microarray analysis demonstrated that 48 differentially expressed miRNAs (18 upregulated and 30 downregulated), of which six miRNAs – miR- 196b- 5p, miR- 339- 3p, miR- 34a- 5p, miR- 376b- 3p, miR- 677- 5p, and miR- 721 – were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR. Conclusions: Urinary exosomal miRNAs showing differences in expression prior to Aβ- plaque deposition were identified. These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications.
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论文发表日期：2021-09-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 233-242 )
英文信息