DOI: 10.1002/ame2.12167
Magnetic resonance imaging under isoflurane anesthesia alters cortical cyclooxygenase- 2 expression and glial cell morphology during sepsis- associated neurological dysfunction in rats
Ibtihel Dhaya1
Marion Griton2
Jan Pieter Konsman3
1.INCIA,Institut de Neurosciences Cognitives et Intégratives d'Aquitaine,CNRS UMR 5287,Bordeaux,France;Univ.Bordeaux,INCIA,UMR 5287,Bordeaux,France;Laboratoire de Neurophysiologie Fonctionnelle et Pathologies,UR/11ES09,Facultédes Sciences Mathématiques,Physiques et Naturelles,Universitéde Tunis El Manar,Tunis,Tunisie2.INCIA,Institut de Neurosciences Cognitives et Intégratives d'Aquitaine,CNRS UMR 5287,Bordeaux,France;Univ.Bordeaux,INCIA,UMR 5287,Bordeaux,France;Service de Réanimation Anesthésie Neurochirurgicale,Centre Hospitalier Universitaire(CHU)de Bordeaux,Bordeaux,France3.INCIA,Institut de Neurosciences Cognitives et Intégratives d'Aquitaine,CNRS UMR 5287,Bordeaux,France;Univ.Bordeaux,INCIA,UMR 5287,Bordeaux,France
摘要：Background: Magnetic resonance imaging (MRI) of rodents combined with histol-ogy allows to determine what mechanisms underlie functional and structural brain changes during sepsis- associated encephalopathy. However, the effects of MRI per-formed in isoflurane- anesthetized rodents on modifications of the blood- brain bar-rier and the production of vasoactive prostaglandins and glia cells, which have been proposed to mediate sepsis- associated brain dysfunction, are unknown.Methods: This study addressed the effect of MRI under isoflurane anesthesia on blood- brain barrier integrity, cyclooxygenase- 2 expression, and glial cell activation during cecal ligature and puncture- induced sepsis- associated brain dysfunction in rats. Results: Cecal ligature and puncture reduced food intake and the righting reflex. MRI under isoflurane anesthesia reduced blood- brain barrier breakdown, decreased cir-cularity of white matter astrocytes, and increased neuronal cyclooxygenase- 2 immu-noreactivity in the cortex 24 hours after laparotomy. In addition, it annihilated cecal ligature and puncture- induced increased circularity of white matter microglia. MRI under isoflurane anesthesia, however, did not alter sepsis- associated perivascular cyclooxygenase- 2 induction. Conclusion: These findings indicate that MRI under isoflurane anesthesia of rodents can modify neurovascular and glial responses and should, therefore, be interpreted with caution.
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论文发表日期：2021-09-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 249-260 )
英文信息