DOI: 10.1002/ame2.12181
New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis
Amir Abdolmaleki1
Cyrus Jalili2
Kamran Mansouri2
Mitra Bakhtiari1
1.Department of Anatomical Sciences,Faculty of Medicine,Kermanshah University of Medical Sciences,Kermanshah,Iran2.Medical Biology Research Center,Health Technology Institute,Kermanshah University of Medical Sciences,Kermanshah,Iran
摘要：Background: Endometriosis can lead to infertility. Since there is no definitive treat-ment for endometriosis, animal modelling seems necessary to examine the possible treatments. Mouse endometrium cannot be separated for endometriosis induc-tion. In addition, transplantation of uterus into the abdominal viscera to induce endometriosis causes organ damage. In this study, we defined a new model of en-dometriosis leading to separability of endometrium and a safe anatomical region for transplantation.Methods: Forty female mice were allocated to 5 groups: 1, sham; 2, allograft uterus transplantation of mice to anterior abdominal wall of mice; 3, allograft uterus trans-plantation of mice to mesentery of mice; 4, xenograft endometrial transplantation of rat to anterior abdominal wall of mice; 5, xenograft endometrial transplantation of rat to mesentery of mice. Adult female rats with a previous pregnancy experience were selected and placed in the vicinity of male rats for 2 weeks to induce estrogen secre-tion and increase endometrial thickness. Results: In the 4th group of animals, compared to sham, the peritoneal concentrations of VEGF- A, TNF- α, NO, MDA, and serum levels of CA- 125 and IL- 37 were increased and total body weight was decreased, while weight and size of endometrial lesions were increased significantly ( P < .05). Genes expression of HOXA10 and HOXA11 were decreased significantly ( P < .05) in groups 2 and 4 compared to sham. Conclusions: Xenograft transplantation of endometrium from rat to anterior abdomi-nal wall of mice can potentially mimic human endometriosis morphologically, histo-logically, and genetically.
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论文发表日期：2021-09-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 268-277 )
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