DOI: 10.1002/ame2.12188
In- depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research
Colton H.Funkhouser1
Liam D.Kirkpatrick1
Robert D.Smith1
Lauren T.Moffatt2
Jeffrey W.Shupp3
Bonnie C.Carney2
1.Firefighters'Burn and Surgical Research Laboratory,MedStar Health Research Institute,Washington,District of Columbia,USA2.Firefighters'Burn and Surgical Research Laboratory,MedStar Health Research Institute,Washington,District of Columbia,USA;Department of Biochemistry and Molecular and Cellular Biology,Georgetown University Medical Center,Washington,District of Columbia,USA;Department of Surgery,Georgetown University School of Medicine,Washington,District of Columbia,USA3.Firefighters'Burn and Surgical Research Laboratory,MedStar Health Research Institute,Washington,District of Columbia,USA;Department of Biochemistry and Molecular and Cellular Biology,Georgetown University Medical Center,Washington,District of Columbia,USA;Department of Surgery,Georgetown University School of Medicine,Washington,District of Columbia,USA;The Burn Center,Department of Surgery,MedStar Washington Hospital Center,Washington,District of Columbia,USA
摘要：Background : Wound healing can result in various outcomes, including hypertrophic scar (HTS). Pigs serve as models to study wound healing as their skin shares physi-ologic similarity with humans. Yorkshire (Yk) and Duroc (Dc) pigs have been used to mimic normal and abnormal wound healing, respectively. The reason behind this dif-ferential healing phenotype was explored here. Methods : Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days. PCR arrays were used to determine differential gene expression. Vancouver Scar Scale (VSS) scores were given. Re- epithelialization was analyzed. H&E, Mason' s trichrome, and immunostains were used to determine cellularity, col-lagen content, and blood vessel density, respectively. Results : Yk wounds heal to a "port wine" HTS, resembling scarring in Fitzpatrick skin types (FST) I-Ⅲ. Dc wounds heal to a dyspigmented, non- pliable HTS, resembling scarring in FST IV– VI. Gene expression during wound healing was differentially regu-lated versus uninjured skin in 40/80 genes, 15 of which differed between breeds. Yk scars had a higher VSS score at all time points. Yk and Dc wounds had equivalent re- epithelialization, collagen disorganization, and blood vessel density. Conclusions : Our findings demonstrate that Dc and Yk pigs can produce HTS. Wound creation and healing were consistent among breeds, and differences in gene expres-sion were not sufficient to explain differences in resulting scar phenotype. Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment' s effectiveness on various skin types.
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论文发表日期：2021-12-25
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 406-417 )
英文信息