DOI: 10.1002/ame2.12217
Royal- jelly- based apitherapy can attenuate damages to male reproductive parameter following nicotine administration
Mahsa Nazar-Zadeh1
Cyrus Jalili2
Amene Nikgoftar Fathi3
Ali Ghanbari1
Mitra Bakhtiari1
1.Department of Anatomical Sciences,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran2.Medical Biology Research Center,Kermanshah University of Medical Sciences,Kermanshah,Iran3.Department of Anatomical Sciences,Medical School,Arak University of Medical Sciences,Arak,Iran
摘要：Background : Nicotine administration can generate severe oxidative stress and lipid peroxidation. Royal jelly, with its antioxidant properties, acts as a scavenger of reac-tive oxygen species. This study describes the apitherapy effects of royal jelly on tes-ticular damage following nicotine administration. Methods : Forty- eight male BALB/c mice were divided into 8 groups ( n = 6): saline, 3 different doses of royal jelly (100, 150, and 200 mg/kg body weight (BW) per day), nicotine (1.5 mg/kg), and 3 different groups of Nic + Roy (1.5 mg/kg of Nic + 100, 150, and 200 mg/kg BW per day of royal jelly). Nicotine was administrated intraperi-toneally, and royal jelly was prescribed orally for 10 consecutive days. Serum levels of hormones (testosterone, luteinizing hormone, and follicle- stimulating hormone), total antioxidant capacity, nitric oxide (NO) status, malondialdehyde levels, sperm DNA fragmentation, sperm parameters, histopathological changes (H&E staining), im-munohistochemistry against apoptotic proteins, and gene expression of Bcl - 2 , p53 , Caspase - 3 , and Nrf2 (real- time PCR) were assessed to evaluate the molecular and his-tological changes. Results : Hormone levels, sperm parameters, and status of antioxidants were de-creased significantly ( p < .05) following nicotine administration. Moreover, royal jelly treatment normalized hormonal and antioxidant characteristics, decreased apoptotic gene expression, increased Nfr2 gene expression, and restored histopathological al-teration to the physiological status significantly ( p < .05). Conclusion : Royal jelly upregulates the antioxidant status, inhibits the mitochondrial- dependent apoptosis pathway, and increases the rate of proliferation. This thera-peutic agent effectively protected the testis against nicotine- associated damages by antioxidant and anti- apoptotic effects.
机标关键词：administrationparameternicotineapitherapyattenuatebaseddamagesfollowing
论文发表日期：2022-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 133-140 )
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