DOI: 10.1002/ame2.12228
A 3D- printable device allowing fast and reproducible longitudinal preparation of mouse intestines
Beckey DeLucia1
Sergey Samorezov2
Megan T.Zangara3
Rachel L.Markley1
Lucas J.Osborn4
Karlee B.Schultz5
Christine McDonald3
Jan Claesen4
1.Department of Cardiovascular and Metabolic Sciences,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA;Center for Microbiome and Human Health,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA2.Department of Biomedical Engineering,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA3.Department of Molecular Medicine,Cleveland Clinic Lerner College of Medicine of Case Western Reserve University,Cleveland,Ohio,USA;Department of Inflammation and Immunity,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA4.Department of Cardiovascular and Metabolic Sciences,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA;Center for Microbiome and Human Health,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA;Department of Molecular Medicine,Cleveland Clinic Lerner College of Medicine of Case Western Reserve University,Cleveland,Ohio,USA5.Department of Cardiovascular and Metabolic Sciences,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA;Center for Microbiome and Human Health,Lerner Research Institute,Cleveland Clinic,Cleveland,Ohio,USA;College of Arts and Sciences,John Carroll University,University Heights,Ohio,USA
摘要：Accurate and reproducible analysis of murine small and large intestinal tissue is key for preclinical models involving intestinal pathology. Currently, there is no easily ac-cessible, standardized method that allows researchers of different skill levels to con-sistently dissect intestines in a time- efficient manner. Here, we describe the design and use of the 3D- printed "Mouse Intestinal Slicing Tool" (MIST), which can be used to longitudinally dissect murine intestines for further analysis. We benchmarked the MIST against a commonly used procedure involving scissors to make a longitudinal cut along the intestines. Use of the MIST halved the time per mouse to prepare the intestines and outperformed alternative methods in smoothness of the cutting edge and overall reproducibility. By sharing the plans for printing the MIST, we hope to contribute a uniformly applicable method for saving time and increasing consistency in studies of the mouse gastrointestinal tract.
机标关键词：devicefastmouseallowingintestineslongitudinalpreparationprintable
论文发表日期：2022-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 189-196 )
英文信息