DOI: 10.1002/ame2.12218
Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1％ ophthalmic atropine sulfate
Alejandra Sánchez-Ríos
Elba Yadira Correa-Gallegos
JoséManuel Medina-Espinoza
Andrea Anaid Navarro-Sanchez
Oscar Olvera-Monta?o
Leopoldo Baiza-Durán
Patricia Mu?oz-Villegas
Medical Affairs Department,Laboratorios Sophia,SA de CV,Zapopan,Jalisco, Mexico
摘要：Background : The objective of this study was to validate an animal model for dry eye during and after the administration of 1％ ophthalmic atropine sulfate (OAS) in New Zealand white (NZW) rabbits. Methods : OAS (1％) was applied three times per day to 30 eyes of 15 healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21, and 30 (OAS group). A second group (n = 5) was used as control. Clinical evalua-tions took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were: Schirmer I test, tear break- up time (TBUT), and corneal fluorescein staining. As sec-ondary endpoints, clinical changes including intraocular pressure, and histopathology were evaluated. Results : While OAS was administered, the Schirmer I test showed a statistically signif-icant reduction for OAS group versus control ( p < 0.001), and versus basal production ( p < 0.001). TBUT showed statistically significant differences between groups (days 3 and 10; p = 0.001) and versus basal values (day 3; p < 0.001). Fluorescein staining showed a statistically significant difference (day 3; p = 0.001). The most frequent clinical finding was conjunctival hyperemia (76.9％ OAS vs. 20％ control). For histo-pathology, all OAS subjects presented some degree of inflammation (86.7％ minimal; 13.3％ mild) whereas the control presented only 30％ minimal inflammation. Goblet cell density showed no difference. Conclusions : The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is main-tained throughout the intervention; it is not a viable model after OAS administration is suspended.
机标关键词：whitemodelatropineduringfollowinginstillationophthalmicpreclinical
论文发表日期：2022-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 266-273 )
英文信息