DOI: 10.1002/ame2.12232
Single intratesticular injection of blood- serum- derived exosomes can potentially alleviate testopathy following testicular torsion
Mona Keivan1
Fatemeh Mansouri Torghabeh2
Samira Davoodi3
Shima Moradi Maryamneghari3
Reza Dadfar4
1.Member of Research Committee,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran2.Department of Physiology Sciences,Medical School,Mashhad University of Medical Sciences,Mashhad,Iran3.Department of Anatomical Sciences,Medical School,Kermanshah University of Medical Sciences,Kermanshah,Iran4.Department of Anatomical Sciences,Faculty of Medicine,AJA University of Medical Sciences,Tehran,Iran
摘要：Background : Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti-i nflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti-i nflammatory effects of blood- serum- derived exosomes in treatment of acute inflammation following TT in mice were evaluated. Materials and Methods : Eighteen male mice were grouped as healthy control, TT, and TT + exosome. TT was induced surgically, and exosomes were extracted from blood serum and administrated by a single intratesticular injection (10 IU). Malondialdehyde (MDA) and Griess assays were used to evaluate the level of oxidative stress. Sperm indices, testosterone (Tes), and apoptotic gene expression (p- 53, Bcl2, and Caspase- 3) were also assessed. H&E and immunohistochemistry (IHC) stainings were used for histopathological investigations. Data analysis was applied by SPSS (v.19) software. Results : Oxidative stress and apoptotic genes expression were increased significantly ( p < 0.05) in TT group compared with control. Sperm parameters and Tes were significantly increased, and expression of apoptotic genes was significantly reduced in TT + exosome group ( p < 0.05). Conclusion : Since the blood- serum- derived exosomes have anti- inflammatory features, the intratesticular application of blood- serum- derived exosomes can be used clinically in acute phase of orchitis following TT to inhibit testicular inflammation.
机标关键词：exosomesinjectionsinglealleviateblood-derivedfollowingintratesticular
论文发表日期：2022-08-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 362-368 )
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