DOI: 10.1002/ame2.12272
Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device
Hong Lian1
Zhuyun Qin2
Mengge Wu3
Peipei Zuo3
Lina Bai4
Minjie Lu4
Lulu Li2
Haitao Zhang5
1.State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China;Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials,Animal Experimental Center,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China2.State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China;Adult Surgical Intensive Care Unit,Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China3.National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine,Fuwai Central-China Hospital,Central-China Subcenter of National Center for Cardiovascular Diseases,Zhengzhou,China4.State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China5.Adult Surgical Intensive Care Unit,Fuwai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China
摘要：Background: To understand the relationship between myocardial contractility and ex-ternal stimuli, detecting ex vivo myocardial contractility is necessary. Methods: We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, volt-ages, and calcium concentrations. Results: The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, con-tractility decreased with an increase in frequency (0.45 ± 0.11– 0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradu-ally increased as the voltage increased from 6 to 10 V (0.14 ± 0.02– 0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17– 1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN). Conclusions: Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
机标关键词：contractilitydevicedetectionmouseintactisolatedmusclemyostation
论文发表日期：2022-10-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 445-452 )
英文信息