DOI: 10.1002/ame2.12280
Human primary muscle stem cells regenerate injured urethral sphincter in athymic rats
Biniam M.Bekele1
Verena Sch?wel-Wolf1
Janine Kieshauer2
Andreas Marg2
Andreas Busjahn3
Sarah Davis4
Gayle Nugent4
Anne-Karoline Ebert5
Simone Spuler1
1.Muscle Research Unit,Experimental and Clinical Research Center,acooperation between the Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association and the Charité,Universit?tsmedizin Berlin,Berlin,Germany;Charité Universit?tsmedizin,Berlin,Germany;Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association,Berlin,Germany;Berlin Institute of Health at Charité Universit?tsmedizin,Belrin,Germany2.Muscle Research Unit,Experimental and Clinical Research Center,acooperation between the Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association and the Charité,Universit?tsmedizin Berlin,Berlin,Germany;Charité Universit?tsmedizin,Berlin,Germany;Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association,Berlin,Germany3.Health Twist GmbH,Berlin,Germany4.Charles River Laboratories,Mattawan,Michigan,USA5.Department of Urology and Pediatric Urology,University Hospital Ulm,Ulm,Germany
摘要：Background: The aim of the study was to demonstrate the efficacy of human muscle stem cells (MuSCs) isolated using innovative technology in restoring internal urinary sphincter function in a preclinical animal model. Methods: Colonies of pure human MuSCs were obtained from muscle biopsy speci-mens. Athymic rats were subjected to internal urethral sphincter damage by electro-cauterization. Five days after injury, 2 × 105 muscle stem cells or medium as control were injected into the area of sphincter damage ( n = 5 in each group). Peak bladder pressure and rise in pressure were chosen as outcome measures. To repeatedly obtain the necessary pressure values, telemetry sensors had been implanted into the rat bladders 10 days prior to injury. Results: There was a highly significant improvement in the ability to build up peak pressure as well as a pressure rise in animals that had received muscle stem cells as compared to control ( p = 0.007) 3 weeks after the cells had been injected. Only mini-mal histologic evidence of scarring was observed in treated rats. Conclusion: Primary human muscle stem cells obtained using innovative technology functionally restore internal urethral sphincter function after injury. Translation into use in clinical settings is foreseeable.
机标关键词：stemhumancellsratsathymicinjuredmuscleprimary
论文发表日期：2022-10-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 453-460 )
英文信息