DOI: 10.1002/ame2.12302
Comparison of the replication and neutralization of different SARS- CoV - 2 Omicron subvariants in vitro
Yaqing Zhang
Qi Lv
Feifei Qi
Fengdi Li
Ran Deng
Xujian Liang
Mingya Liu
Yiwei Yan
Linlin Bao
Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases,NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,China;National Center of Technology Innovation for Animal Model,Beijing, China
摘要：Background : New Omicron subvariants are emerging rapidly from BA.1 to BA.4 and BA.5. Their pathogenicity has changed from that of wild- type (WH- 09) and Omicron variants have over time become globally dominant. The spike proteins of BA.4 and BA.5 that serve as the target for vaccine-i nduced neutralizing antibodies have also changed compared to the previous subvariants, which is likely to cause immune es-cape and the reduction of the protective effect of the vaccine. Our study addresses the above issues and provides a basis for formulating relevant prevention and control strategies.Methods : We collected cellular supernatant and cell lysates and measured the viral titers, viral RNA loads, and E subgenomic RNA (E sgRNA) loads in different Omicron subvariants grown in Vero E6 cells, using WH- 09 and Delta variants as a reference. Additionally, we evaluated the in vitro neutralizing activity of different Omicron sub-variants and compared it to the WH- 09 and Delta variants using macaque sera with different types of immunity. Results : As the SARS- CoV- 2 evolved into Omicron BA.1, the replication ability in vitro began to decrease. Then with the emergence of new subvariants, the replication ability gradually recovered and became stable in the BA.4 and BA.5 subvariants. In WH- 09-i nactivated vaccine sera, geometric mean titers of neutralization antibodies against different Omicron subvariants declined by 3.7～15.4- fold compared to those against WH- 09. In Delta-i nactivated vaccine sera, geometric mean titers of neutrali-zation antibodies against Omicron subvariants declined by 3.1～7.4- fold compared to those against Delta. Conclusion : According to the findings of this research, the replication efficiency of all Omicron subvariants declined compared with WH- 09 and Delta variants, and was lower in BA.1 than in other Omicron subvariants. After two doses of inactivated (WH- 09 or Delta) vaccine, cross- neutralizing activities against various Omicron subvariants were seen despite a decline in neutralizing titers.
机标关键词：differentvitrocomparisonneutralizationomicronreplicationsars-subvariants
论文发表日期：2023-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 51-56 )
英文信息