DOI: 10.1002/ame2.12294
Histological and magnetic resonance imaging assessment of Liqoseal in a spinal in vivo pig model
Emma M.H.Slot1
Wilhelmina Bergmann2
Ahmet Kinaci3
Bart de Boer4
Nizar Moayeri3
Saskia Redegeld5
Sander van Thoor5
Tristan P.C.van Doormaal6
1.Department of Neurology and Neurosurgery,University Medical Center Utrecht,Utrecht,the Netherlands;Department of Translational Neuroscience,Brain Center,University Medical Center Utrecht,Utrecht,the Netherlands2.Department of Biomolecular Health Sciences,Division of Pathology,Faculty of Veterinary Medicine,Utrecht University,Utrecht,the Netherlands3.Department of Neurology and Neurosurgery,University Medical Center Utrecht,Utrecht,the Netherlands4.Department of Neurosurgery,Elizabeth TweeSteden ziekenhuis,Tilburg,the Netherlands5.Brain Technology Institute,Utrecht,the Netherlands6.Department of Neurology and Neurosurgery,University Medical Center Utrecht,Utrecht,the Netherlands;Department of Translational Neuroscience,Brain Center,University Medical Center Utrecht,Utrecht,the Netherlands;Department of Neurosurgery,Clinical Neuroscience Center,University Hospital Zurich,Zurich,Switzerland
摘要：Background : Liqoseal (Polyganics, B.V.) is a dural sealant patch for preventing postop-erative cerebrospinal fluid (CSF) leakage. It has been extensively tested preclinically and CE ( Conformité Européenne ) approved for human use after a first cranial in- human study. However, the safety of Liqoseal for spinal application is still unknown. The aim of this study was to assess the safety of spinal Liqoseal application compared with cranial application using histology and magnetic resonance imaging characteristics.Methods : Eight female Dutch Landrace pigs underwent laminectomy, durotomy with standard suturing and Liqoseal application. Three control animals underwent the same procedure without sealant application. The histological characteristics and im-aging characteristics of animals with similar survival times were compared to data from a previous cranial porcine model. Results : Similar foreign body reactions were observed in spinal and cranial dura. The foreign body reaction consisted of neutrophils and reactive fibroblasts in the first 3 days, changing to a chronic granulomatous inflammatory reaction with an increasing number of macrophages and lymphocytes and the formation of a fibroblast layer on the dura by day 7. Mean Liqoseal plus dura thickness reached a maximum of 1.2 mm (range 0.7– 2.0 mm) at day 7. Conclusion : The spinal dural histological reaction to Liqoseal during the first 7 days was similar to the cranial dural reaction. Liqoseal did not swell significantly in both application areas over time. Given the current lack of a safe and effective dural sealant for spinal ap-plication, we propose that an in- human safety study of Liqoseal is the logical next step.
机标关键词：imagingmodelvivoassessmenthistologicalliqosealmagneticresonance
论文发表日期：2023-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 74-80 )