DOI: 10.1002/ame2.12303
A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles
Yao He1
Jie Yang2
Fengying Hu3
Min Liao2
Yuru Nie3
Xiaoxia Zhu2
Tao Zhang4
Keer Song5
Qinxi Li1
Xiaojie Li1
Chenghan Mei1
Zhe Wu3
Qiang Lu2
Zhihui Zhong1
1.Laboratory of Nonhuman Primate Disease Modeling Research,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu,China2.Department of Medical Ultrasound,West China Hospital,Sichuan University,Chengdu,China3.School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu,China4.School of Bioscience and Technology,Chengdu Medical College,Chengdu,China5.Franklin College of Arts and Science,University of Georgia,Athens,Georgia,USA
摘要：Background:We aimed to prepare a non-invasive,reproducible,and controllable rat model of intracerebral hemorrhage with focused ultrasound(FUS).Methods:A rat intracerebral hemorrhage(ICH)model was established by combining FUS and microbubbles(μBs),and edaravone was used to verify whether the free radi-cal scavenger had a protective effect on the model.The brain tissue of each group was sectioned to observe the gross histology,blood-brain barrier(BBB)permeability,cerebral infarction volume,and histopathological changes.Results:Compared with the FUS group,the BBB permeability was significantly in-creased in the FUS+μBs(F&B)group(p=0.0021).The second coronal slice in the F&B group had an obvious hemorrhage lesion,and the FUS+μBs+edaravone(F&B&E)group had smaller hemorrhage areas;however,ICH did not occur in the FUS group.The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group(p=0.0030)and F&B&E group(p=0.0208).HE staining results showed that nerve fibrinolysis,neuronal necrosis,microglia production,and erythro-cytes were found in both the F&B group and the F&B&E group,but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.Conclusions:A rat ICH model was successfully prepared using the μBs assisted FUS treatment,and edaravone had a therapeutic effect on this model.This model can be used to study the pathophysiological mechanism of ICH-related diseases and in pre-clinical research on related new drugs.
机标关键词：ultrasoundmethodmodelcombiningfocusedhemorrhageintracerebralmicrobubbles
论文发表日期：2023-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 103-110 )
英文信息