DOI: 10.1002/ame2.12260
Anti-inflammatory effects of amarogentin on 2,4-dinitrochlorobenzene-induced atopic dermatitis-like mice and in HaCat cells
Qian Zhang1
Hanlin Wang2
Cheng Ran3
Yansi Lyu4
Fei Li1
Yihang Yao2
Shaojun Xing5
Li Wang4
Si Chen2
1.Department of Dermatology,The Sixth Affiliated Hospital of Shenzhen University and Huazhong University of Science and Technology Union Shenzhen Hospital,Shenzhen,China2.Department of Immunology,Shenzhen University Health Science Center,Shenzhen,China3.Department of Otolaryngology,Affiliate Hospital of Hebei University,Baoding,China4.Department of Dermatology,Shenzhen University General Hospital,Shenzhen University,Shenzhen,China5.Department of Pathogen Biology,Shenzhen University Health Science Center,Shenzhen,China
摘要：Background:Amarogentin(AMA)is a secoiridoid glycoside extracted from Swertia and Gentiana roots and exhibits many biological effects such as antioxidative,anti-inflammatory,and antitumor activities.Atopic dermatitis(AD)is a chronic inflam-matory skin disease caused by disorders in the regulation of multiple inflammatory cytokines.No effective cure has been found for AD now.Methods:We constructed the HaCat and splenocyte model and tested the inhibitory effect of AMA on IL-4,IL-6,and IL-13 secretions using enzyme-linked immunosorb-ent assay(ELISA).The AD mouse model was constructed and treated with AMA,the severity of skin lesions was observed,epidermal tissue was collected,and epidermal thickness and mast cell infiltration were observed using hematoxylin and eosin and toluidine blue staining,respectively.The expression of kallikrein-related peptidase 7(KLK7)and filaggrin(FLG)was detected using immunostaining and Western blot anal-ysis.The mRNA expression of KLK7 and FLG was detected using quantitative poly-merase chain reaction(qPCR).Blood immunoglobulin E(IgE)secretion was detected.Results:AMA inhibited IL-6 secreted by tumor necrosis factor(TNF)-a-induced HaCaT cells and reduced IL-4 and IL-13 secreted by phytohemagglutinin(PHA)-induced pri-mary cells in the mice spleen.It was found that the treatment of AMA with 2,4-din itrochlorobenzene-induced AD-like mice could promote the recovery of dermatitis,reduce the score of dermatitis severity and the scratching frequency,treat the skin lesions,reduce the epidermal thickness,decrease the infiltration of mast cells,reduce the IgE level in serum,decrease the expression levels of AD-related cytokines,in-crease protein and mRNA expression of FLG,and reduce the protein and mRNA ex-pression of KLK7 in the skin tissues of AD-like mice.Conclusion:In conclusion,AMA inhibits inflammatory response at the cellular level,and AMA reduces the validation response of specific dermatitis mice,relieves pruri-tus,and repairs the damaged skin barrier.
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论文发表日期：2023-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 255-265 )
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