DOI: 10.1002/ame2.12325
Validation of a surgical model for posthepatectomy liver failure in rats
Andrea Lund
Michelle Meier
Kasper Jarlhelt Andersen
Marie Ingemann Pedersen
Anders Riegels Knudsen
Jakob Kirkeg?rd
Frank Viborg Mortensen
Aarhus University Hospital,Department of Upper Gastrointestinal and Hepato-Pancreato-Biliary Surgery,Aarhus N,Denmark
摘要：Background:The upper limit for liver resections in rats is approximately 90％.In the early postoperative phase,mortality increases.The aim of the present study was to validate the rat model of 90％partial hepatectomy(PH)as a model of post-hepatectomy liver failure(PHLF).Further,we wanted to test a quantitative scoring system as a detector of lethal outcomes caused by PHLF in rats.Methods:Sixty-eight rats were randomized to 90％PH,sham operation,or no sur-gery.Further,block randomization was performed based on time of euthanization:12,24,or 48 h after surgery.A general distress score(GDS)≥10 during the day or ≥6 at midnight prompted early euthanization and classification as nonsurvivor.Animals euthanized as planned were classified as survivors.During euthanization,blood and liver tissue were collected,and liver-specific biochemistry was evaluated.Results:Based on the biochemical results,all animals subjected to 90％PH expe-rienced PHLF.Seventeen rats were euthanized due to irreversible PHLF.The GDS increased for nonsurvivors within 12-18 h after surgery.The mean time for euthaniza-tion was 27 h after surgery.Conclusion:Based on the GDS and liver-specific biochemistry,we concluded that the model of 90％PH seems to be a proper model for investigating PHLF in rats.As a high GDS is associated with increased mortality,the GDS appears to be valuable in detect-ing lethal outcomes caused by PHLF in rats.
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论文发表日期：2023-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 266-273 )
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