DOI: 10.1002/ame2.12338
Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
Yiming Ding1
Xulei Dai2
Miaoye Bao2
Yuanming Xing3
Junhui Liu4
Sihai Zhao2
Enqi Liu2
Zuyi Yuan5
Liang Bai2
1.Department of Laboratory Animal Science,School of Basic Medical Sciences,Xi'an Jiaotong University Health Science Center,Xi'an,China;Department of Cardiology,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,China;Institute of Cardiovascular Science,Translational Medicine Institute,Xi'an Jiaotong University Health Science Center,Xi'an,China2.Department of Laboratory Animal Science,School of Basic Medical Sciences,Xi'an Jiaotong University Health Science Center,Xi'an,China;Institute of Cardiovascular Science,Translational Medicine Institute,Xi'an Jiaotong University Health Science Center,Xi'an,China3.Department of Cardiology,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,China;Institute of Cardiovascular Science,Translational Medicine Institute,Xi'an Jiaotong University Health Science Center,Xi'an,China4.Department of Clinical Laboratory,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,China5.Department of Cardiology,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,China
摘要：Background:Nonalcoholic fatty liver disease(NAFLD)is the main reason for cirrho-sis and hepatocellular carcinoma.As a starting point for NAFLD,the treatment of nonalcoholic fatty liver(NAFL)is receiving increasing attention.Mice fed a high-fat diet(HFD)and hereditary leptin deficiency(ob/ob)mice are important NAFL animal models.However,the comparison of these mouse models with human NAFL is still unclear.Methods:In this study,HFD-fed mice and ob/ob mice were used as NAFL animal mod-els.Liver histopathological characteristics were compared,and liver transcriptome from both mouse models was performed using RNA sequencing(RNA-seq).RNA-seq data obtained from the livers of NAFL patients was downloaded from the GEO database.Global gene expression profiles in the livers were further analyzed using functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway.Results:Our results showed that the biochemical parameters of both mouse models and human NAFL were similar.Compared with HFD-fed mice,ob/ob mice were more similar in histologic appearance to NAFL patients.The liver transcriptome charac-teristics partly overlapped in mice and humans.Furthermore,in the NAFL pathway,most genes showed similar trends in mice and humans,thus demonstrating that both types of mice can be used as models for basic research on NAFL,considering the differences.Conclusion:Our findings show that HFD-fed mice and ob/ob mice can mimic human NAFL partly in pathophysiological process.The comparative analysis of liver tran-scriptome profile in mouse models and human NAFL presented here provides insights into the molecular characteristics across these NAFL models.
机标关键词：withmicediseasefattyhepatichumanslivernonalcoholic
论文发表日期：2023-08-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 317-328 )
英文信息