DOI: 10.1002/ame2.12329
A combined pig model to determine the net absorption of volatile fatty acids in the large intestine under different levels of crude fiber
Shengjun Zhao1
Liangkang Lv2
Taotao Wu1
Zhi Feng1
Qiang Li1
Long Lei1
Zhengya Liu1
Hui Zhang1
Ying Ren1
1.Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan,China2.State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing,China
摘要：Background:This study aimed to develop a combined model to quantify the net ab-sorption of volatile fatty acids(VFA)in the large intestine(LI)of pigs.Methods:Fifteen female growing pigs(Duroc×Large White×Landrace)were ranked by body weight(30±2.1 kg)on day 0 and assigned to one of three treatments,namely the basal diet containing different crude fiber(CF)levels(LCF:3.0%CF,MCF:4.5%CF,and HCF:6.0%CF).The pigs were implanted with the terminal ileum fistula and the cannulation of the ileal mesenteric vein(IMV),portal vein(PV),and left femoral artery(LFA)from days 6 to 7.[13 C]-Labeled VFA and P-aminohippuric acid were constantly perfused into the terminal ileum fistula and the cannulation of the IMV(day 15),re-spectively.Blood samples were collected from the PV and the LFA during perfusion(5 h),and LI samples were collected.Results:The net flux of[12 C]-acetic acid in the PV was greater for LCF versus MCF(p = 0.045),but no difference was observed in the net flux of[12 C]-propionic acid(p = 0.505)and[12 C]-butyric acid(p = 0.35)in the PV among treatments.The deposi-tion of[12 C]-acetic acid in the LI was greater for LCF versus MCF(p = 0.014),whereas the deposition of[12 C]-propionic acid(p = 0.007)and[12 C]-butyric acid(p = 0.037)in the LI was greater for LCF versus HCF.Conclusions:In conclusion,this pig model was found conducive to study the net ab-sorption of VFAs in the LI,and LCF had more net absorption of VFAs in the LI than MCF and HCF.
机标关键词：differentlevelsmodellargefiberabsorptionacidscombined
论文发表日期：2023-08-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 375-380 )
英文信息