DOI: 10.1002/ame2.12321
Identification of optimal reference genes in golden Syrian hamster with ethanol-and palmitoleic acid-induced acute pancreatitis using quantitative real-time polymerase chain reaction
Jinxin Miao1
Le Kang1
Tianfeng Lan2
Jianyao Wang2
Siqing Wu1
Yifan Jia1
Xia Xue3
Haoran Guo2
Pengju Wang2
Yan Li1
1.Academy of Chinese Medicine Science,Henan University of Chinese Medicine,Zhengzhou,China2.Sino-British Research Centre for Molecular Oncology,National Center for International Research in Cell and Gene Therapy,School of Basic Medical Sciences,Academy of Medical Sciences,Zhengzhou University,Zhengzhou,China3.Henan Key Laboratory of Helicobacter pylori and Microbiota and Gastrointestinal Cancer,Marshall Medical Research Center,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou,China
摘要：Background : Acute pancreatitis (AP) is a severe disorder that leads to high morbidity and mortality. Appropriate reference genes are important for gene analysis in AP. This study sought to study the expression stability of several reference genes in the golden Syrian hamster, a model of AP.Methods : AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol (1.35 g/kg) and palmitoleic acid (2 mg/kg). The expression of candidate genes, including Actb , Gapdh , Eef2 , Ywhaz , Rps18 , Hprt1 , Tubb , Rpl13a , Nono , and B2m , in hamster pancreas at different time points (1, 3, 6, 9, and 24 h) posttreatment was analyzed using quantitative polymerase chain reaction. The expression stability of these genes was calculated using BestKeeper, Comprehensive Delta CT, NormFinder, and geNorm algorithms and RefFinder software. Results : Our results show that the expression of these reference genes fluctuated during AP, of which Ywhaz and Gapdh were the most stable genes, whereas Tubb , Eef2 , and Actb were the least stable genes. Furthermore, these genes were used to normalize the expression of TNF- α messenger ribonucleic acid in inflamed pancreas. Conclusions : In conclusion, Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.
机标关键词：identificationpolymerasequantitativereferencereal-timegoldenreactionoptimal
论文发表日期：2023-12-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 609-618 )
英文信息