DOI: 10.1002/ame2.12386
Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA-Seq approach
Roa'a H.S.Yosief1
Iqbal M.Lone1
Aharon Nachshon2
Heinz Himmelbauer3
Irit Gat-Viks2
Fuad A.Iraqi1
1.Department of Clinical Microbiology and Immunology,Sackler Faculty of Medicine,Tel-Aviv University,Tel-Aviv,Israel2.School of Molecular Cell Biology and Biotechnology,George S.Wise Faculty of Life Sciences,Tel Aviv University,Tel Aviv,Israel3.Institute of Computational Biology,Department of Biotechnology,University of Natural Resources and Life Sciences,Muthgasse 18,1190 Vienna,Austria
摘要：Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene expression.
Methods:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with Af.Liver tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was performed.The GENE-E analysis platform was used to generate a clustered heat map matrix.
Results:Significant variation in body weight changes between CC lines was ob-served.Hepatic gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight changes.Interestingly,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome 8.
Conclusions:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af.As a next step,eQTL analysis will be performed for our RNA-Seq data.Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.
机标关键词：aspergillusfumigatusgeneticmiceapproachcollaborativecrossidentifying
论文发表日期：2024-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 36-47 )
英文信息