DOI: 10.1002/ame2.12378
Development of a new cerebral ischemia reperfusion model of Mongolian gerbils and standardized evaluation system
Ying Wu1
Caijiao Hu1
Zhihui Li1
Feiyang Li1
Jianyi Lv1
Meng Guo1
Xin Liu1
Changlong Li1
Xueyun Huo1
Zhenwen Chen1
Lifeng Yang2
Xiaoyan Du1
1.School of Basic Medical Sciences,Capital Medical University,Beijing,China;Laboratory for Clinical Medicine,Capital Medical University,Beijing,China2.College of Veterinary Medicine,China Agricultural University,Beijing,China
摘要：Background:The Mongolian gerbil is an excellent laboratory animal for preparing the cerebral ischemia model due to its inherent deficiency in the circle of Willis.However,the low incidence and unpredictability of symptoms are caused by numerous complex variant types of the circle.Additionally,the lack of an evaluation system for the cer-ebral ischemia/reperfusion(I/R)model of gerbils has shackled the application of this model.
Methods:We created a symptom-oriented principle and detailed neurobehavioral scoring criteria.At different time points of reperfusion,we analyzed the alteration in locomotion by rotarod test and grip force score,infarct volume by triphenyltetrazo-lium chloride(TTC)staining,neuron loss using Nissl staining,and histological charac-teristics using hematoxylin-eosin(H&E)straining.
Results:With a successful model rate of 56%,32 of the 57 gerbils operated by our method harbored typical features of cerebral I/R injury,and the mortality rate in the male gerbils was significantly higher than that in the female gerbils.The suc-cessfully prepared I/R gerbils demonstrated a significant reduction in motility and grip strength at 1 day after reperfusion;formed obvious infarction;exhibited typi-cal pathological features,such as tissue edema,neuronal atrophy and death,and vacuolated structures;and were partially recovered with the extension of reperfu-sion time.
Conclusion:This study developed a new method for the unilateral common carotid artery ligation I/R model of gerbil and established a standardized evaluation system for this model,which could provide a new cerebral I/R model of gerbils with more practical applications.
机标关键词：developmentevaluationischemiasystemmodelcerebralgerbilsmongolian
论文发表日期：2024-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 48-55 )
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