DOI: 10.1002/ame2.12388
The efficacy of vitamin E in preventing arthrofibrosis after joint replacement
Yingfang Fan1
Jean Yuh2
Sashank Lekkala2
Mehmet D.Asik1
Andrew Thomson2
Madeline McCanne2
Mark A.Randolph3
Antonia F.Chen4
Ebru Oral1
1.Harris Orthopaedic Laboratory,Department of Orthopaedic Surgery,Massachusetts General Hospital,Boston,Massachusetts,USA;Department of Orthopaedic Surgery,Harvard Medical School,Boston,Massachusetts,USA2.Harris Orthopaedic Laboratory,Department of Orthopaedic Surgery,Massachusetts General Hospital,Boston,Massachusetts,USA3.Department of Orthopaedic Surgery,Harvard Medical School,Boston,Massachusetts,USA;Department of Surgery,Harvard Medical School,Boston,Massachusetts,USA4.Department of Orthopaedic Surgery,Brigham and Women's Hospital,Boston,Massachusetts,USA
摘要：Background:Arthrofibrosis is a joint disorder characterized by excessive scar forma-tion in the joint tissues.Vitamin E is an antioxidant with potential anti-fibroblastic effect.The aim of this study was to establish an arthrofibrosis rat model after joint replacement and assess the effects of vitamin E supplementation on joint fibrosis.
Methods:We simulated knee replacement in 16 male Sprague-Dawley rats.We immobilized the surgical leg with a suture in full flexion.The control groups were killed at 2 and 12 weeks(n=5 per group),and the test group was supplemented daily with vitamin E(0.2 mg/mL)in their drinking water for 12 weeks(n=6).We per-formed histological staining to investigate the presence and severity of arthrofibrosis.Immunofluorescent staining and α2-macroglobulin(α2M)enzyme-linked immuno-sorbent assay(ELISA)were used to assess local and systemic inflammation.Static weight bearing(total internal reflection)and range of motion(ROM)were collected for functional assessment.
Results:The ROM and weight-bearing symmetry decreased after the procedure and recovered slowly with still significant deficit at the end of the study for both groups.Histological analysis confirmed fibrosis in both lateral and posterior peri-articular tissue.Vitamin E supplementation showed a moderate anti-inflammatory effect on the local and systemic levels.The vitamin E group exhibited significant improvement in ROM and weight-bearing symmetry at day 84 compared to the control group.
Conclusions:This model is viable for simulating arthrofibrosis after joint replacement.Vitamin E may benefit postsurgical arthrofibrosis,and further studies are needed for dosing requirements.
机标关键词：jointafterarthrofibrosisefficacypreventingreplacementvitamin
论文发表日期：2024-04-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 145-155 )
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