DOI: 10.1002/ame2.12369
Jiaohong pills attenuate neuroinflammation and amyloid-β protein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway
Hong Zhang
Weiyan Cai
Lijinchuan Dong
Qing Yang
Qi Li
Qingsen Ran
Li Liu
Yajie Wang
Yujie Li
Xiaogang Weng
Xiaoxin Zhu
Ying Chen
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China
摘要：Background:Jiaohong pills (JHP) consist of Pericarpium Zanthoxyli (PZ) and Radix Rehmanniae ,two herbs that have been extensively investigated over many years due to their potential protective effects against cognitive decline and memory im-pairment.However,the precise mechanisms underlying the beneficial effects remain elusive.Here,research studies were conducted to investigate and validate the thera-peutic effects of JHP on Alzheimer's disease.
Methods:BV-2 cell inflammation was induced by lipopolysaccharide.AD mice were administered amyloid-β (Aβ).Behavioral experiments were used to evaluate learn-ing and memory ability.The levels of nitric oxide (NO),tumor necrosis factor-alpha (TNF-α),interleukin-1β (IL-1β),and interleukin-10 (IL-10) were detected using enzyme-linked immunosorbent assay (ELISA).The protein expressions of inducible nitric oxide synthase (iNOS) and the phosphorylation level of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) were detected using Western blot.Nissl staining was used to detect neuronal degeneration.
Results:The results demonstrated that an alcoholic extract of PZ significantly de-creased the levels of NO,IL-1β,TNF-α,and iNOS; increased the expression level of IL-10; and significantly decreased the phosphorylation levels of MAPK and NF-κB.These inhibitory effects were further confirmed in the AD mouse model.Meanwhile,JHP improved learning and memory function in AD mice,reduced neuronal damage,and enriched the Nissl bodies in the hippocampus.Moreover,IL-1β and TNF-α in the cortex were significantly downregulated after JHP administration,whereas IL-10 showed increased expression.
Conclusions:It was found that JHP reduced neuroinflammatory response in AD mice by targeting the MAPK/NF-κB signaling pathway.
机标关键词：cognitivepathwayactivatedamyloid-attenuatedeficitseurofactor
论文发表日期：2024-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 222-233 )
英文信息