DOI: 10.1002/ame2.12440
Material basis and pharmacodynamic mechanism of YangshenDingzhi granules in the intervention of viral pneumonia: Based on serum pharmacochemistry and network pharmacology
Huirong Xu1
Meiyue Dong2
Ruikun Du3
Chengcheng Zhang4
Zinuo Chen2
Guangyu Tian1
Qinghua Cui3
Kejian Li1
1.College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan,China2.Innovative Institute of Chinese Medicine and Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,China3.Innovative Institute of Chinese Medicine and Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,China;Qingdao Academy of Chinese Medical Sciences,Shandong University of Traditional Chinese Medicine,Qingdao,China4.College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,China
摘要：Background:YangshenDingzhi granules (YSDZ) are clinically effective in preventing and treating COVID-19.The present study elucidates the underlying mechanism of YSDZ intervention in viral pneumonia by employing serum pharmacochemistry and network pharmacology.
Methods:The chemical constituents of YSDZ in the blood were examined using ultra-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spec-trometry (UPLC-Q-Exactive Orbitrap MS).Potential protein targets were obtained from the SwissTargetPrediction database,and the target genes associated with viral pneumonia were identified using GeneCards,DisGeNET,and Online Mendelian Inheritance in Man (OMIM) databases.The intersection of blood component-related targets and disease-related targets was determined using Venny 2.1.Protein-protein interaction networks were constructed using the STRING database.The Metascape database was employed to perform enrichment analyses of Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling path-ways for the targets,while the Cytoscape 3.9.1 software was utilized to construct drug-component-disease-target-pathway networks.Further,in vitro and in vivo ex-periments were performed to establish the therapeutic effectiveness of YSDZ against viral pneumonia.
Results:Fifteen compounds and 124 targets linked to viral pneumonia were detected in serum.Among these,MAPK1,MAPK3,AKT1,EGFR,and TNF play significant roles.In vitro tests revealed that the medicated serum suppressed the replication of H1N1,RSV,and SARS-CoV-2 replicon.Further,in vivo testing analysis shows that YSDZ de-creases the viral load in the lungs of mice infected with RSV and H1N1.
Conclusion:The chemical constituents of YSDZ in the blood may elicit therapeutic effects against viral pneumonia by targeting multiple proteins and pathways.
机标关键词：chemistrymechanismmaterialnetworkcochbasisbasedgranules
论文发表日期：2024-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：16( 259-274 )
英文信息