DOI: 10.1002/ame2.12411
Considerations for the use of porcine organ donation models in preclinical organ donor intervention research
Frazer I.Heinis1
Shaheed Merani2
Nicholas W.Markin3
Kim F.Duncan4
Michael J.Moulton4
Lance Fristoe5
William E.Thorell6
Raechel A.Sherrick7
Tami R.Wells8
Matthew T.Andrews1
Marian Urban4
1.School of Natural Resources,Institute of Agriculture and Natural Resources,University of Nebraska-Lincoln,Lincoln,Nebraska,USA2.Division of Transplantation and Vascular Surgery,Department of Surgery,University of Nebraska Medical Center,Omaha,Nebraska,USA3.Department of Anesthesiology,University of Nebraska Medical Center,Omaha,Nebraska,USA4.Division of Cardiothoracic Surgery,Department of Surgery,University of Nebraska Medical Center,Omaha,Nebraska,USA5.Clinical Perfusion,Nebraska Medicine-Nebraska Medical Center,Omaha,Nebraska,USA6.Department of Neurosurgery,University of Nebraska Medical Center,Omaha,Nebraska,USA7.Nutrition and Health Sciences,College of Education and Human Sciences,University of Nebraska-Lincoln,Lincoln,Nebraska,USA8.Department of Comparative Medicine,University of Nebraska Medical Center,Omaha,Nebraska,USA
摘要：Use of animal models in preclinical transplant research is essential to the optimization of human allografts for clinical transplantation.Animal models of organ donation and preservation help to advance and improve technical elements of solid organ recovery and facilitate research of ischemia-reperfusion injury,organ preservation strategies,and future donor-based interventions.Important considerations include cost,public opinion regarding the conduct of animal research,translational value,and relevance of the animal model for clinical practice.We present an overview of two porcine mod-els of organ donation: donation following brain death (DBD) and donation following circulatory death (DCD).The cardiovascular anatomy and physiology of pigs closely resembles those of humans,making this species the most appropriate for pre-clinical research.Pigs are also considered a potential source of organs for human heart and kidney xenotransplantation.It is imperative to minimize animal loss during procedures that are surgically complex.We present our experience with these models and de-scribe in detail the use cases,procedural approach,challenges,alternatives,and limi-tations of each model.
机标关键词：researchmodelsdonorconsiderationsdonationinterventionorganporcine
论文发表日期：2024-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：14( 283-296 )
英文信息