DOI: 10.1002/ame2.12449
Establishment of a humanized ST6GAL1 mouse model for influenza research
Lyu Chao1
Han Feng2
Gao Qian3
Lv Limin2
Lu Ziwei1
Lu Shuangshuang1
Li Xiaoyan1
Hu Yuechao3
Yang Mengjie4
Zhao Yingze3
Liu Jun3
Lu Xuancheng1
Duo Shuguang2
1.National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Chinese Center for Disease Control and Prevention,Beijing,China2.Institute of Zoology,Chinese Academy of Sciences,Beijing,China3.NHC Key Laboratory of Biosafety,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China4.NHC Key Laboratory of Biosafety,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China;Zhejiang Provincial Key Laboratory of Medical Genetics,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China
摘要：Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.
Methods:Humanized fragments,consisting of the endothelial cell-specific K18 pro-moter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the ovi-ducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propa-gation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical (IHC) analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.
Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as car-rying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1 gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.
Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.
机标关键词：researchmousemodelestablishmenthumanizedinfluenza
论文发表日期：2024-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 337-346 )
英文信息