DOI: 10.1002/ame2.12447
A rat model of multicompartmental traumatic injury and hemorrhagic shock induces bone marrow dysfunction and profound anemia
Lauren S.Kelly1
Jennifer A.Munley1
Erick E.Pons1
Kolenkode B.Kannan1
Elizabeth M.Whitley2
Letitia E.Bible1
Philip A.Efron1
Alicia M.Mohr1
1.Department of Surgery and Sepsis and Critical Illness Research Center,University of Florida College of Medicine,Gainesville,Florida,USA2.Pathogenesis,LLC,Gainesville,Florida,USA
摘要：Background:Severe trauma is associated with systemic inflammation and organ dys-function.Preclinical rodent trauma models are the mainstay of postinjury research but have been criticized for not fully replicating severe human trauma.The aim of this study was to create a rat model of multicompartmental injury which recreates profound traumatic injury.
Methods:Male Sprague-Dawley rats were subjected to unilateral lung contusion and hemorrhagic shock (LCHS),multicompartmental polytrauma (PT) (unilateral lung con-tusion,hemorrhagic shock,cecectomy,bifemoral pseudofracture),or naïve controls.Weight,plasma toll-like receptor 4 (TLR4),hemoglobin,spleen to body weight ratio,bone marrow (BM) erythroid progenitor (CFU-GEMM,BFU-E,and CFU-E) growth,plasma granulocyte colony-stimulating factor (G-CSF) and right lung histologic injury were assessed on day 7,with significance defined as p values <0.05 (*).
Results:Polytrauma resulted in markedly more profound inhibition of weight gain compared to LCHS (p=0.0002) along with elevated plasma TLR4 (p<0.0001),lower hemoglobin (p<0.0001),and enlarged spleen to body weight ratios (p=0.004).Both LCHS and PT demonstrated suppression of CFU-E and BFU-E growth compared to naïve (p<0.03,p<0.01).Plasma G-CSF was elevated in PT compared to both naïve and LCHS (p<0.0001,p=0.02).LCHS and PT demonstrated significant histologic right lung injury with poor alveolar wall integrity and interstitial edema.
Conclusions:Multicompartmental injury as described here establishes a reproduc-ible model of multicompartmental injury with worsened anemia,splenic tissue en-largement,weight loss,and increased inflammatory activity compared to a less severe model.This may serve as a more effective model to recreate profound traumatic in-jury to replicate the human inflammatory response postinjury.
机标关键词：multimodelanemiabonecompartmentdysfunctionhemorrhagicinduces
论文发表日期：2024-06-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 367-376 )
英文信息