DOI: 10.1002/ame2.12460
An ethically guided preclinical device for phenotyping H2 production in laboratory rodents
Victor Pascal-Moussellard
Emilie Boucher
Stéphane Tanguy
Philippe Cinquin
Pierre-Alain Barraud
Chloé Davin
Cordélia Salomez-Ihl
Dalil Hannani
François Boucher
Jean-Pierre Alcaraz
University Grenoble Alpes,CNRS,UMR 5525,VetAgro Sup,Grenoble INP,TIMC,Grenoble,France
摘要：Background:Dihydrogen (H2 ) is produced endogenously by the intestinal microbiota through the fermentation of diet carbohydrates.Over the past few years,numer-ous studies have demonstrated the significant therapeutic potential of H2 in various pathophysiological contexts,making the characterization of its production in labora-tory species of major preclinical importance.
Methods:This study proposes an innovative solution to accurately monitor H2 pro-duction in free-moving rodents while respecting animal welfare standards.The devel-oped device consisted of a wire rodent cage placed inside an airtight chamber in which the air quality was maintained,and the H2 concentration was continuously analyzed.After the airtightness and efficiency of the systems used to control and maintain air quality in the chamber were checked,tests were carried out on rats and mice with different metabolic phenotypes,over 12 min to 1-h experiments and repeatedly.H2 production rates (HPR) were obtained using an easy calculation algorithm based on a first-order moving average.
Results:HPR in hyperphagic Zucker rats was found to be twice as high as in control Wistar rats,respectively,2.64 and 1.27 nmol.s-1 per animal.In addition,the ingestion of inulin,a dietary fiber,stimulated H2 production in mice.HPRs were 0.46 nmol.s-1 for animals under control diet and 1.99 nmol.s-1 for animals under inulin diet.
Conclusions:The proposed device coupled with our algorithm enables fine analysis of the metabolic phenotype of laboratory rats or mice with regard to their endogenous H2 production.
机标关键词：productiondeviceethicallyguidedlaboratoryphenotypingpreclinicalrodents
论文发表日期：2024-08-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 553-561 )
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