DOI: 10.1002/ame2.12450
Influence of ear tags on the results of body composition analysis in mice
He Liu1
Yinghua Zhang2
Peng Zhang3
Weiping Teng1
Zhongyan Shan1
Yushu Li1
Dan Wang3
1.Department of Endocrinology and Metabolism and the Institute of Endocrinology,NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases,The First Hospital of China Medical University,Shenyang,China2.School of Public Health China Medical University,China Medical University,Shenyang,China3.Shenyang Food and Drug Inspection Institute,Shenyang,China
摘要：Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.
Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.
Results:(1) The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2) There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3) The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.
Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.
机标关键词：analysistagsmicebodycompositioninfluenceresults
论文发表日期：2024-08-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 578-583 )
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